Lysosomes are known as the recycling centers of the cell, breaking down and recycling cellular waste, macromolecules, and organelles to maintain cell health. As we age, lysosomal function can decline, leading to an accumulation of cellular debris and dysfunctional proteins, which is thought to contribute to the aging process and age-related diseases. Emerging research suggests that enhancing lysosomal function may improve cellular health and longevity. By measuring lysosomal activity, we can gain insights into how these organelles influence aging and identify potential therapeutic targets. For example, assays that measure the degradation rate of specific substrates can indicate lysosomal efficiency, while imaging techniques can reveal changes in lysosome number and distribution within cells. Given the complexity of lysosomal functions, it is important to establish robust and sensitive protocols for their measurements.

• Design and refine experimental protocols to assess lysosomal functions, leveraging existing methods for mammalian sample analysis;
• Process samples for mass spectrometry analysis to study lysosomal components;
• C. elegans cultures;
• Perform imaging analyses, including the use of confocal microscopy and staining techniques, to investigate lysosomal properties.

We are looking for an enthusiastic technician with at least 3 years work experience and with:

• An HLO, Bachelor’s or Master’s degree in Life Sciences, Biochemistry, Medical Biology, or a related field;
• Experience in mass spectrometry analysis and worm culture is appreciated but not required;
• Proficiency in both oral and written English required to perform this position. Excellent communication and organizational skills and the ability to work in a dynamic group are essential.

• A flying start to your career in scientific research.
• Plenty of room for personal development with a wide range of training & development of academic skills
• Working on large-scale and own research, with motivated colleagues
• A contract for 6 months with the prospect of extension for the duration.
• Salary scale: 7M: € 2.975 tot € 4.214 bruto (based on a fulltime workweek), depending on qualifications and experience.
• Additional benefits including an 8.3% end-of-year bonus and 8% holiday pay. Calculate your net salary here.
• Your pension is accrued at BeFrank. A modern, understandeble en fair pension
• Excellent accessibility with public transportation and reimbursement of a large portion of your travel expenses. Additionally, we have plenty of parking spaces at the AMC location and a good bicycle scheme.

Amsterdam UMC

Laboratory Genetic Metabolic Disease (Lab GMD) of Amsterdam UMC, location AMC combines diagnostic and research specialized in metabolism in a broad context, ranging from rare inherited metabolic diseases to cancer and age-related metabolic diseases. Within the Lab GMD, the Translational Metabolism group led by Prof. Dr. Riekelt Houtkooper focuses on different topics, among them, the molecular regulation of metabolic disease and how disturbances in metabolic pathways that underlie metabolic disease can be targeted as a therapy. The goal of our research is to translate our findings into future therapies that help prevent or cure disease.