PhD candidate 'Structure and dynamics of nuclear RNA foci in myotonic dystrophy type 1 and 2 (DC5)'

The ENTRY-DM Doctoral Network, funded under the prestigious Marie Skłodowska-Curie Actions – Doctoral Networks (MSCA-DN) programme, is offering 14 fully funded PhD positions across leading European institutions. Join ENTRY-DM and contribute to the next wave of RNA-based therapeutics for myotonic dystrophy, a field at the cutting edge of precision medicine and molecular therapies. Be part of a transformative research network that will shape the future of rare disease treatment.

ENTRY-DM is an ambitious interdisciplinary and intersectoral training programme that will equip the next generation of researchers with the expertise to develop novel oligonucleotide-based therapies for myotonic dystrophy. This programme brings together leading academic institutions, innovative biotech companies, and patient advocacy groups, forming a unique collaborative environment for doctoral training at the intersection of fundamental science, translational medicine, and clinical application.

Myotonic dystrophy (DM) is the most common inherited muscular dystrophy in adults. It is a complex multi-systemic disorder, impacting not only skeletal and cardiac muscle but also the nervous system, among others. Despite recent advances in understanding the genetic and molecular mechanisms of DM, no effective treatment is currently available. The use of antisense oligonucleotides (ASOs) to counteract the disease-causing mutant RNA has faced delivery challenges and insufficient therapeutic efficacy. The development of clinically viable RNA-targeted therapeutics requires overcoming key scientific and technological hurdles, including poor ASO biodistribution, limited penetration of critical tissues, and the lack of reliable biomarkers to monitor disease progression and treatment response.

ENTRY-DM aims to transform the therapeutic landscape for Myotonic dystrophy by training a new generation of creative PhD researchers in a structured, interdisciplinary research programme. The project will focus on three major research objectives:

• Development of innovative disease models and exploring disease mechanisms.
• Optimising ASO-based therapies
• Defining clinical biomarkers for clinical trials.

PhD researchers will investigate genotype-phenotype correlations and RNA dynamics, leveraging cutting-edge genomics, bioinformatics, stem cell research, bioengineering of innovative 3D disease models, medicine chemistry, advanced imaging techniques, as well as neuropsychology approaches and clinical applications, in a truly multidisciplinary project.

To achieve these goals, ENTRY-DM has assembled a consortium of leading European research institutions and biotech companies, providing a truly international and intersectoral research environment. The 9 academic and 2 non-academic beneficiaries include renowned universities and research centres in France, Spain, Italy, the Netherlands, Germany, and Poland, complemented by 13 associated partners, including patient organisations and biotech companies. Each PhD candidate will be hosted in a dynamic, multi-disciplinary and international setting, benefiting from inter-sectoral secondments, world-class supervision, and specialised training in advanced research techniques, innovation, and career development.

As a PhD candidate you will write scientific publications, resulting in a thesis. You will present your research at local, national, and international meetings, work collaboratively with other PhD candidates and scientists, and engage in research in a stimulating environment, including travel to other European institutes.

We are looking for a motivated and ambitious PhD candidate with a strong interest in RNA therapeutics and microscopic imaging. The ideal candidate will have a passion for molecular and cellular techniques, cell culture, and image analysis. A degree in molecular cell biology or a related field is required, along with practical experience in molecular and cellular techniques and cell culture. Prior experience with microscopy is highly desirable.

Candidates with well-developed leadership qualities, strong motivation, and initiative are highly valued. Excellent analytical and problem-solving skills, as well as a commitment to advancing research, are essential. Effective communication and teamwork abilities are crucial for collaboration within our group. Proficiency in English is mandatory; knowledge of or willingness to learn Dutch is a plus.

Responsibilities include developing and standardizing iPSC-based cell models and microscopic assays for relevant disease readouts.

Doctoral candidates will spend time on secondment during their 36 month employment contracts. Anticipated secondments for this position are:

• Universitat de Valencia, Ruben Artero (Valencia, Spain). Use of antagomirs to upregulate MBNL protein expression and study of effects on foci dynamics.
• Centre d’Etudes des Cellules Souches, Sandrine Baghdoyan, (Evry, France). hiPSC differentiation protocols into 2D and 3D relevant disease models.

Starting date is 1 September 2025. At Radboud university medical center, you build on your future. We are committed to providing the best care, education, and research. And we are true to our word, because we help you develop and seize opportunities and give you the room to grow. As an employer, we believe that employees should feel vital and happy at work in all stages of life. We are also committed to creating a healthy and safe working environment. Our employment conditions contribute to that. What we offer:

• Upon commencement of employment, you will start at scale 10A, step 0 (€ 3.017 based on a full-time appointment). Over a maximum period of 4 years, you will progress to scale 10A, step 3 (€3.824 based on a full-time appointment). You will also receive an 8% holiday allowance, an 8.3% end-of-year bonus, and a 47% to 72% bonus for working unsocial hours.
• From 1 July 2025 an increase in salary with 3%.
• 176 vacation hours per year based on a 36-hour working week.

The ENTRY-DM consortium includes 9 academic and 2 non-academic beneficiaries from 6 European countries, along with leading associated partners from academia, industry, and patient organisations. PhD positions are available at the following institutions:

• Inserm (Paris, France) – 2 positions.
• Universitat de València (Valencia, Spain) – 2 positions.
• Institut de Bioenginyeria de Catalunya (Barcelona, Spain) – 1 position.
• Università di Roma Tor Vergata (Rome, Italy) – 1 position.
• Radboud University Medical Centre (Nijmegen, The Netherlands) – 2 positions.
• Ludwig-Maximilians-Universität München (Munich, Germany) – 1 position.
• Adam Mickiewicz University in Poznań (Poznan, Poland) – 1 position.
• Centre d’Etudes des Cellules Souches (Evry, France) – 1 position.
• Genartis (Genova, Italy) – 1 position.
• Université Paris Cité (Paris, France) – 1 position.
• Consejo Superior de Investigaciones Científicas (Barcelona, Spain) – 1 position.

By combining high-resolution imaging and the use of DMPK and CUG repeat-targeting drugs, DC5 will resolve and understand the structure, formation and dissolution of C/CUG RNA MBNL-containing foci, a hallmark in disease manifestation of DM1 and DM2.

Using hiPSC-derived cell models (myogenic precursors, neurons, astrocytes) and established drugs (e.g. ASOs and small molecules), DC5 will address the following specific aims:

• Characterization of the high resolution structure of nuclear (C/CUG)n RNA foci using combined in situ hybridization and high-resolution immunofluorescent microscopy (CSLM and STORM).
• Investigation of expression effects of DMPK (DM1) and CNBP1 (DM2) transcripts, including length of the C/CUG repeat, and MBNL1/MBNL2 protein on foci number, size, structure and content.
• Study foci dynamics under proliferating and differentiating (post-mitotic) conditions, in absence and presence of therapeutics.

Radboudumc
Welcome to Radboud university medical center (Radboudumc), where scientific breakthroughs are born through the curiosity and passion of our collaborating researchers in a vibrant environment. We believe trust and excitement are crucial elements to achieving our goals, and we approach our research as teams and with the utmost rigor to make a significant impact on health and healthcare. Our researchers are driven by their fascination with the biological, psychological, and sociological mechanisms underlying health and healthcare. They collaborate with partners from all over the world to improve health outcomes for all. Radboudumc unites patient care, research, education, and corporate learning, which allows us to approach our mission to shape the health and healthcare of the future in an innovative and person-centered way.

Our ambition is to lead the way in the pursuit of prevention, sustainability, meaningful care. We believe that through our research, we can significantly improve the health and well-being of society. Join us on our mission to make a difference in healthcare. Become a part of our community of gifted researchers, professionals or patient partners who are dedicated to making a real impact on population health and healthcare.

Read what it is like to do a PhD at the Radboud University Medical Center.

Any questions? Or wondering what it is like to work at Radboudumc? Then email to Rick Wansink, Associate Professor. Use the Apply button to submit your application.

Application procedure
Interested candidates should upload:

• A detailed CV (maximum two pages), specifying educational background (name of university/universities, year degree(s) obtained, title(s) of degree(s)), qualifications, work experience, skills, research interests, list of publications (if any) and the names and contact details of two referees.
• A motivation letter (maximum two pages), describing the candidate’s interest in the network, previous research experience, and current research interest outlining the fit to the desired PhD Project.
• If you are planning to apply to more than one position in the network, please limit your applications to three positions maximum (from DC1 to DC14) and please provide a separate document detailing which positions you have applied for.
• Copy of passport or National Identify Card.
• Copies of academic certificates (BSc, MSc, and any other relevant diplomas), translated into English, including expected final grade and date. Proof of English proficiency, demonstrated by IELTS, TOEFL, or a university certification confirming that the degree or MSc thesis was conducted in English.
• Two recommendation letters (maximum 1 page each). Only documents in English will be accepted.

Applications failing to include the requested documentation, who do not indicate the preferred projects or do not meet the eligibility criteria, will not be considered.

Any questions? Or wondering what it is like to work at Radboudumc? Then email to Rick Wansink, Associate Professor. Use the Apply button to submit your application.

Application procedure
Interested candidates should upload:

• A detailed CV (maximum two pages), specifying educational background (name of university/universities, year degree(s) obtained, title(s) of degree(s)), qualifications, work experience, skills, research interests, list of publications (if any) and the names and contact details of two referees.
• A motivation letter (maximum two pages), describing the candidate’s interest in the network, previous research experience, and current research interest outlining the fit to the desired PhD Project.
• If you are planning to apply to more than one position in the network, please limit your applications to three positions maximum (from DC1 to DC14) and please provide a separate document detailing which positions you have applied for.
• Copy of passport or National Identify Card.
• Copies of academic certificates (BSc, MSc, and any other relevant diplomas), translated into English, including expected final grade and date. Proof of English proficiency, demonstrated by IELTS, TOEFL, or a university certification confirming that the degree or MSc thesis was conducted in English.
• Two recommendation letters (maximum 1 page each). Only documents in English will be accepted.

Applications failing to include the requested documentation, who do not indicate the preferred projects or do not meet the eligibility criteria, will not be considered.