The technician will support a PhD student in a Dutch Cancer Society funded project studying whether GARP (glycoprotein-A repetition predominant) plays a key role in immune suppression in head and neck cancer.

With the current treatment arsenal, half of the 900,000 patients that will yearly receive the diagnosis head and neck squamous cell carcinoma, will not survive 5 years. In the Netherlands, immunotherapy with immune checkpoint inhibitors targeting PD-1 have been introduced in 2018 for the treatment of patients with recurrent or metastatic head and neck cancer. While several remarkable responses have been observed, the majority of patients do not benefit this treatment, while side-effects can be severe and treatments are costly.

A clinical challenge is to improve efficacy of (immuno)therapy and identify biomarkers that can predict, prior to treatment, whether a patient is likely or unlikely to respond to treatment. Additionally, unravelling GARPs mechanistic role in suppressing the immune system could provide leads for the development of novel combination therapies in the future. This project is a collaboration between the Dept. of Otolaryngology | Head & Neck Surgery and the Dept. of Medical Oncology at Amsterdam UMC (location VUMC).

• As a technician on this project, you will be working closely with a PhD student on this project in an internationally renowned research environment;
• You will be able to learn from (technical) staff within the HNCBI laboratory and CCA;
• Together with the PhD student, you will set-up, plan and perform experiments and perform data analysis. We have weekly Project meetings to discuss these with the team;
• Techniques include: spatial transcriptomics, multi-parameter flow cytometry, multiplex fluorescent immunohistochemistry and functional immune assays;
• You will help train Bachelor and Master students during their practical internship.

• You have a Bsc degree in laboratory science, or you will receive your degree in 2023;
• You have a background in immunology or molecular biology with an interest in oncology;
• You have experience in basic immunology and/or cell biology techniques (experience with any of the techniques described in the previous section is highly valued, but not required);
• You can work independently, but you are also a team player;
• You are eager to learn new techniques and skills and you have good communication skills.

• We offer an initial contract for 12 months for 32 hours/week, that can be extended (for another 12 or 24 months after evaluation).
• Salary scale technician (scale 7 or 8) depending on qualifications and experience: € 2,711- € 4,095 gross, based on 32 hours/week.
• Effective of November 2023, a 4% salary increase will occur.

In addition we offer:

• An 8.3% end-of-year bonus and 8% holiday pay.
• Free and unlimited access to our online learning environment: GoodHabitz.
• Pension is accrued at the ABP. Amsterdam UMC contributes 70% of the premium.
• We reimburse 75% of you public transport commuting costs. Would you rather bike? We have a good bike arrangement in place.
• You can choose to participate in the active staff association, which organizes fun activities and events.

Amsterdam UMC

The Head and Neck Cancer Biology and Immunology (HNCBI) laboratory is embedded within the department of Otolaryngology | Head & Neck Surgery at Amsterdam UMC, location VUmc.

Prof. C. René Leemans is the Head of the department and the HNCBI laboratory is chaired by Prof. Ruud Brakenhoff. Our scientific aim is to improve the diagnosis and treatment of patients with head and neck squamous cell carcinoma. The lab contains around 15 scientists (senior staff, PhD students, technicians). Dr. Rieneke van de Ven leads the tumor-immunology research within the HNCBI lab.

Our research group is embedded in two Amsterdam UMC research Institutes: Cancer Center Amsterdam (Cancer Biology and Immunology theme) and Amsterdam Institute for Infection and Immunity (Tumor Immunology theme).

Amsterdam UMC is an inspiring and professional working environment, in which we encourage employees to develop their talents and skills. For this purpose, we offer career development opportunities and trainings.