As a research technician at the Leiden hiPSC Centre of LUMC, you'll be responsible for generating and genetically modifying human induced pluripotent stem cells (hiPSCs) for researchers within and outside LUMC. You'll work closely with the senior researcher to produce and characterize genetically modified cell lines, utilizing advanced techniques such as molecular cloning, PCR analysis, immunofluorescence microscopy, and flow cytometry. Additionally, you'll contribute to maintaining the stem cell database, differentiating hiPSCs into specific somatic cell types, and writing and optimizing protocols. You'll also have the opportunity to play a supporting role in the practical courses on "stem cells" and "gene-editing" organized once or twice a year, helping with material preparation and assisting in the lab. Your top 3 tasks are:

•          Producing and characterizing genetically modified hiPSC lines, including isogenic controls, knockouts, and reporters

•          Isolating, culturing, and reprogramming primary cells into hiPSCs, and expanding and managing hiPSC stocks

•          Performing various analyses, including (dd)PCR, immunofluorescence microscopy, and flow cytometry

As the ideal candidate for this position, you're a motivated and proactive professional who strives for accuracy and quality in your work. You enjoy working both independently and as part of a team, and adapt easily to an international environment. You're service-oriented, communicate clearly in English, and take initiative to improve processes. Furthermore, you have:

•          A BSc degree in biological sciences, biochemistry, cell biology, or a related field, with a minimum of 2 years of relevant work experience

•          Demonstrable experience with cell culture and CRISPR/Cas9 gene-editing techniques; experience with pluripotent stem cell culture is a plus

•          Practical knowledge of molecular biological techniques such as molecular cloning, (dd)PCR, and preferably also immunofluorescence microscopy and flow cytometr

·         As a research technician, your salary ranges from € 2,975 to € 4,214 gross per month (scale 7M, CAO UMC). These figures are based on a full-time position.

·         The procedure involves several stages, including two interview rounds. The first interview will be with Babet van er Vaart (and Christian Freund, if available), followed by a second interview with the rest of the team. Detailed information about the next steps in the application process will follow soon

Your career at the LUMC
Driven by health; that's our mission. This applies not only to our patients but also to our employees. With Future@Work, we guarantee a pleasant working environment and provide room for development. In order to be able to continue to learn and develop, we offer internal and external training. You are also entitled to an end-of-year bonus (8,3%), holiday allowance, sports budget, bicycle scheme and reimbursement for commuting expenses. Furthermore, as an employee of LUMC, you are also affiliated with the ABP pension fund. This means that 70% of your pension premium is paid by LUMC, leaving you with a higher net salary. Nice, right?

LUMC

At the Leiden University Medical Center, we continuously work on improving patient care. We invest in groundbreaking, international research and work with the latest equipment. Together with our team of doctors, medical specialists, teachers, academic researchers and supporting staff, we aim for the best quality in health care, education and international research. And we need you to realize our goals!

Leiden hiPSC Centre,

The Leiden hiPSC Centre, established in 2011 as part of LUMC, is a leading facility for generating, characterizing, and genetically modifying human induced pluripotent stem cells (hiPSCs). Located in the Mirai House at the Leiden Bio Science Park, the center is part of the Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) and provides comprehensive services to researchers both domestically and internationally.

Working at the Leiden hiPSC Centre offers a dynamic and international environment where you'll collaborate with a motivated team of researchers. You'll have access to cutting-edge technologies and contribute to groundbreaking research and education, making your work both challenging and socially relevant.